Advantages of using Mycoplasmacheck for mycoplasma PCR detection over in-house PCR kits The graph demonstrates the superiority of mycoplasma PCR over culture-based or bioluminescence-based (Mycoalert) techniques in analysing cell lines for mycoplasma (adapted from Cytotechnology (2014) 66:861–873)įig. Comparison of three different methods for detection of mycoplasma in cell cultures. Besides severely reducing the hands-on time involved in in-house testing, outsourcing the task of mycoplasma detection offers distinct benefits that cannot be matched by do-it-yourself kits (Fig 2).įig. These mycoplasma, all found in the European Pharmacopoeia 2.6.7., are readily detectable with Mycoplasmacheck.Īs a qPCR-based assay, the technique is more accurate and sensitive than traditional microbial culture methods and bioluminescence-based methods for mycoplasma detection (Fig1). List of mycoplasma species frequently detected in cell cultures. With its standardised workflows, we can rapidly and reliably detect all common mycoplasma species. Mycoplasma contamination adversely affects 35% of the world’s cell lines.Īs signs of mycoplasma are easy to miss, only regular monitoring of cell cultures can ensure that research results are valid and reproducible. Reliably detect all common mycoplasma species PDF document serving as an independent certificate of results suitable for direct submission to journals or granting agencies.The user can compile a PDF document online at any time to view the results from any specific testing period.Data report indicating mycoplasma status, as well as any PCR inhibitors found in the sample.Easy sample drop-off at any Collection Point or ship separately.Exclusively licensed from the German Cancer Research Center, (Deutsches Krebsforschungszentrum, DKFZ), Heidelberg, Germany.Use of positive and negative controls to monitor test performance.Highly specific test that identifies ten copies of mycoplasma per reaction with no cross-reactivity to E.Highly sensitive assay capable of detecting 1 international unit (IU) per PCR of the World Health Organization’s (WHO) international standard for mycoplasma DNA (Please see application note).Identification of more than 100 additional mollicutes. yeatsii, Spiroplasma citri and Acholeplasma laidlawii Detection of all mycoplasma species listed in the European Pharmacopoeia 2.6.7: M.Full-service package offered by Mycoplasmacheck:
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